Limit of two collections, no more than a total of 2-5mm of the distal tail removed over all collections. ![]() More than one sample over the life of the animal.Tailing at this age is potentially painful and should be avoided if possible.Use of a systemic analgesic given prior to tail snipping is required.Sampling of animals greater than 24 days of age.Sampling of neonatal mice under 10 days of age (consult with OAR veterinarian).The following are examples of acceptable deviations if appropriate justification is provided in the Animal Protocol: Note: Any procedure(s), other than a one-time collection in animals between 10-24 days of age, must be described and justified in the Animal Protocol. blood or fur) from scissors and sanitize after each snipping Return animal to its cage and monitor for bleeding for at least 5 minutes.If continuous pressure does not stop the bleeding, utilize a chemical cautery agent (e.g.Apply pressure to the cut portion of the tail with gauze until bleeding has stopped.Place tail tip into a tissue collection tube.Minimize the amount of tissue removed - 2 mm of distal tail has been identified as sufficient tissue to perform multiple PCR reactions 2.Snip tail with sanitized sharp scissors or disposable blade.Gently, but securely, restrain animal (manual or mechanical).Note: Better quality DNA and higher DNA yield has been reported from tail snips at 18 days of age or younger, due to a lower percentage of ossified sample.anesthesia or analgesia recommended but not required. ![]() This policy is approved by the University of Iowa Institutional Animal Care and Use Committee (IACUC). This policy is intended for use by research staff and Office of Animal Resources staff approved to perform this procedure on an Animal Protocol. Purpose: The intent of this policy is to describe procedures required for tail tissue collection in rodents for genetic analysis. An exception to a Policy must be described and justified in the Animal Protocol and approved by the full IACUC at a convened monthly meeting. ![]() Policy: The IACUC has provided a set of guidance documents (Policies, Guidelines, and Informational Sheets) for use when planning animal procedures at the University of Iowa.
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